Mouse Collagen I ELISA Kit (A327030)

Mouse Collagen I ELISA Kit

Mouse Collagen I ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of mouse Collagen I in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Mouse Collagen I ELISA Kit (A327030) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Collagen I in serum, plasma, and other biological fluids. An antibody specific for Collagen I has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Collagen I present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Collagen I Antibody, which binds the captured Collagen I present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Collagen I captured in each well. The concentration of Collagen I can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

56.25 pg/ml

93.75-6000 pg/ml

3h 30m

No significant cross-reactivity or interference between mouse Collagen I and analogues was observed.

Intra-assay CV: 5.05%, Inter-assay CV: 5.72%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Alpha 1 type I collagen, Alpha 2 type I collagen, alpha 2 type I procollagen, alpha 2(I) procollagen, alpha 2(I)-collagen, Alpha-1 type I collagen, alpha1(I) procollagen, CO1A1_HUMAN, COL1A1, COL1A2, collagen alpha 1 chain type I, Collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, Collagen I alpha 1 polypeptide, Collagen I alpha 2 polypeptide, collagen of skin, tendon and bone, alpha-1 chain, collagen of skin, tendon and bone, alpha-2 chain, Collagen type I alpha 1, Collagen type I alpha 2, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I procollagen, type I procollagen alpha 1 chain