Mouse CACNA1E ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CACNA1E in tissue homogenates, cell lysates or other biological fluids.

Assay Type

Sandwich (quantitative)

Principle of Assay

Mouse CACNA1E ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of mouse CACNA1E in mouse. An antibody specific for CACNA1E has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CACNA1E present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CACNA1E Antibody, which binds the captured CACNA1E present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CACNA1E captured in each well. The concentration of CACNA1E can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colourmetric

Instrument

Colorimetric Microplate Reader

Sample Type

Tissue homogenates, cell lysates or other biological fluids.

Sensitivity

0.121 ng/ml

Product Range

0.312-20 ng/ml

Assay Time

1 - 4.5 Hours

Reactivity

Mouse

Recovery

Sample Type	n	Range	Average
Serum	5	83% - 100%	91%
EDTA Plasma	5	90% - 98%	94%
Heparin Plasma	5	89% - 102%	95%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	83-110%	79-100%	90-98%	82-108%
EDTA Plasma (n=5)	90-98%	90-105%	73-109%	87-91%
Heparin Plasma (n=5)	88-103%	77-105%	95-98%	87-110%

Precision

Intra-assay: CV < 10%, Inter-assay: CV < 12%

General Notes

Information online is representative. Always refer to the Product Manual inside the kit.

Storage

Store at +4°C. Do not use past expiration date!

Synonyms

alpha-1 polypeptide, BII, Brain calcium channel II, CAC 1E, CAC1E, CAC1E_HUMAN, CACH 6, CACH6, Cacnl1 a6, CACNL1A6, Calcium channel, Calcium channel, L type, alpha 1 polypeptide, isoform 6, calcium channel, R type, Cav 2.3, Cav2.3, Cchra 1, Cchra1, isoform 6, L type, Voltage dependent R type calcium channel subunit alpha 1E, Voltage gated calcium channel subunit alpha Cav2.3, Voltage-dependent R-type calcium channel subunit alpha-1E, Voltage-gated calcium channel subunit alpha Cav2.3

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.