Mouse Adiponectin ELISA Kit (A513)

$675
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Mouse Adiponectin ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This Adiponectin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Adiponectin Antibody. Standards or samples are then added to the microtire plate wells and Adiponectin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Adiponectin present in the sample, Anti-Adiponectin Antibody (Biotin) is added to each well to "sandwich" the Adiponectin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Adiponectin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 10 pg/ml
Product Range
312-20,000 pg/ml
Reactivity
Mouse
Recovery
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, adipocyte-specific secretory protein, Adiponectin precursor, adiponectin, C1Q and collagen domain containing, Adipoq, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPO_HUMAN, ADIPQTL1, ADPN, APM 1, apM-1, APM1, C1q and collagen domain-containing protein, GBP28, Gelatin binding protein , Gelatin binding protein 28, Gelatin-binding protein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Mouse Adiponectin ELISA Kit (A513)? Please let us know so that we can list the citation on this page.

Alternative products to Mouse Adiponectin ELISA Kit (A513)

Top