Monkey Factor XII ELISA Kit (A327058)

Monkey Factor XII ELISA Kit

Monkey Factor XII ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey Factor XII in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Monkey Factor XII ELISA Kit (A327058) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Factor XII in serum, plasma, and other biological fluids. An antibody specific for Factor XII has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Factor XII present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Factor XII Antibody, which binds the captured Factor XII present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Factor XII captured in each well. The concentration of Factor XII can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.94 ng/ml

1.56-100 ng/ml

Monkey

No significant cross-reactivity or interference between monkey Factor XII and analogues was observed.

Intra-assay CV: 4.8%, Inter-assay CV: 4.39%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Beta factor XIIa part 1, Beta factor XIIa part 2, Beta-factor XIIa part 1, Beta-factor XIIa part 2, Coagulation factor XII, Coagulation factor XIIa heavy chain, Coagulation factor XIIa light chain, F12, F12 deficiency, FA12_HUMAN, Factor XII deficiency, HAE3, HAEX, HAF, HAF deficiency, Hageman factor