Anti-MMP25 Antibody (A91145)

Name: Anti-MMP25 Antibody
Brand: Antibodies.com
SKU: A91145
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to MMP25 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MMP25 Antibody

Description

Rabbit polyclonal antibody to MMP25.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 321-539 of human MMP25 (NP_071913.1).

Sequence

FDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Leukolysin, matrix metallopeptidase 25, matrix metallopeptidase like 1, matrix metalloproteinase 20, Matrix Metalloproteinase 25, Matrix metalloproteinase 25 precursor, Matrix metalloproteinase-25, Membrane type 6 Matrix Metalloproteinase, Membrane type Matrix Metalloproteinase 6, Membrane-type matrix metalloproteinase 6, Membrane-type-6 matrix metalloproteinase, MMP 25, MMP-25, MMP20, MMP20A, MMP25_HUMAN, MMPL1, MT-MMP 6, MT6-MMP, MT6MMP, MTMMP6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-MMP25 Antibody (A91145)

Western blot analysis of extracts of various cell lines, using Anti-MMP25 Antibody (A91145) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-MMP25 Antibody (A91145)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-MMP25 Antibody (A91145) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-MMP25 Antibody (A91145)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-MMP25 Antibody (A91145) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MMP25 Antibody (A91145)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-MMP25 Antibody (A91145) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.