Anti-MMP12 Antibody (A11474)

Name: Anti-MMP12 Antibody
Brand: Antibodies.com
SKU: A11474
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to MMP12 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MMP12 Antibody

Description

Rabbit polyclonal antibody to MMP12.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MMP12 (NP_002417.2).

Sequence

DEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 3.4.24.65, HME, Macrophage elastase, Macrophage metalloelastase, Macrophage metaloelastase, Matrix metallopeptidase 12 (macrophage elastase), Matrix metalloprotease 12, Matrix metalloproteinase-12, ME, MGC138506, MME, MMP 12, MMP-12, MMP12_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MMP12 Antibody (A11474)

Western blot analysis of extracts of A-549 cells, using Anti-MMP12 Antibody (A11474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-MMP12 Antibody (A11474)

Immunofluorescence analysis of A-549 cells using Anti-MMP12 Antibody (A11474) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-MMP12 Antibody (A11474)

Immunofluorescence analysis of NIH/3T3 cells using Anti-MMP12 Antibody (A11474) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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