Anti-MLH1 Antibody [ARC53543] (A308155)

Name: Anti-MLH1 Antibody [ARC53543]
Brand: Antibodies.com
SKU: A308155
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53543] antibody to MLH1 for WB and IHC.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-MLH1 Antibody [ARC53543]

Description

Rabbit monoclonal [ARC53543] antibody to MLH1.

Applications

WB, IHC

Dilutions

WB: 1:10,000-1:70,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MLH1 (NP_000240.1).

Sequence

PGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53543

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC, HNPCC 2, HNPCC2, MGC5172, MLH 1, MLH1_HUMAN, MutL homolog 1, MutL homolog 1 (E. coli), MutL homolog 1 colon cancer nonpolyposis type 2, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1, MutL, E. coli, homolog of, 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of various cell lines, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of mouse brain, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts from normal (control) and MLH1 knockout (KO) HeLa cells, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Immunohistochemistry - Anti-MLH1 Antibody [ARC53543] (A308155)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MLH1 expression (negative control sample) using Anti-MLH1 Antibody [ARC53543] (A308155) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.