Anti-MiTF Antibody [ARC0660] (A306202)

Name: Anti-MiTF Antibody [ARC0660]
Brand: Antibodies.com
SKU: A306202
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0660] antibody to MiTF for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MiTF Antibody [ARC0660]

Description

Rabbit monoclonal [ARC0660] antibody to MiTF.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 387-509 of human MITF (O75030).

Sequence

HLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0660

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

59 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

bHLHe32, Class E basic helix-loop-helix protein 32, CMM8, Homolog of mouse microphthalmia, Mi, Microphthalmia associated transcription factor, Microphthalmia, mouse, homolog of, Microphthalmia-associated transcription factor, mitfa, MITF_HUMAN, nacre, WS2, WS2A, z3A.1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-MiTF Antibody [ARC0660] (A306202)

Western blot analysis of extracts of various cell lines, using Anti-MiTF Antibody [ARC0660] (A306202) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-MiTF Antibody [ARC0660] (A306202)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-MiTF Antibody [ARC0660] (A306202) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-MiTF Antibody [ARC0660] (A306202)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-MiTF Antibody [ARC0660] (A306202) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MiTF Antibody [ARC0660] (A306202)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-MiTF Antibody [ARC0660] (A306202) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.