Anti-Milk Fat Globule 1 Antibody (A90406)

Name: Anti-Milk Fat Globule 1 Antibody
Brand: Antibodies.com
SKU: A90406
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Milk Fat Globule 1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Milk Fat Globule 1 Antibody

Description

Rabbit polyclonal antibody to Milk Fat Globule 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human MFGE8 (NP_001108086.1).

Sequence

MPRPRLLAALCGALLCAPSLLVALDICSKNPCHNGGLCEEISQEVRGDVFPSYTCTCLKGYAGNHCETKCVEPLGLENGNIANSQIAASSVRVTFLGLQHWVPELARLNRAGMVNAWTPSSNDDNPWIQVNLLRRMWVTGVVTQGASRLASHEYLKAFKVAYSLNGHEFDFIHDVNKKHKEFVGNWNKNAVHVNLFETPV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BA46, Breast epithelial antigen BA46, Contains Medin , EDIL1, HMFG, hP47, HsT19888, Lactadherin, Lactadherin [Precursor] , Medin, MFG E8, MFG-E8, MFGE8, MFGM, MFGM_HUMAN, Milk fat globule EGF factor 8, Milk fat globule EGF factor 8 protein, Milk fat globule-EGF factor 8, O acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, Sperm associated antigen 10, Sperm surface protein hP47

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-Milk Fat Globule 1 Antibody (A90406)

Western blot analysis of extracts of various cell lines, using Anti-Milk Fat Globule 1 Antibody (A90406) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Milk Fat Globule 1 Antibody (A90406)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Milk Fat Globule 1 Antibody (A90406) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Milk Fat Globule 1 Antibody (A90406)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Milk Fat Globule 1 Antibody (A90406) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Milk Fat Globule 1 Antibody (A90406)

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Anti-Milk Fat Globule 1 Antibody (A90406) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.