Anti-MIF Antibody (A309405)

Name: Anti-MIF Antibody
Brand: Antibodies.com
SKU: A309405
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit polyclonal antibody to MIF for WB and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-MIF Antibody

Description

Rabbit polyclonal antibody to MIF.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human MIF.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

12 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GIF, GLIF, Glycosylation inhibiting factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor, Macrophage migration inhibitory factor (glycosylation-inhibiting factor), MIF protein, MIF_HUMAN, MMIF, Phenylpyruvate tautomerase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-MIF Antibody (A309405)

Western blot analysis of various lysates, using Anti-MIF Antibody (A309405) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Western Blot - Anti-MIF Antibody (A309405)

Western blot analysis of extracts from wild type(WT) and MIF knockdown (KD) 293T(KD) cells, using Anti-MIF Antibody (A309405) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 3: Immunofluorescence - Anti-MIF Antibody (A309405)

Immunofluorescence analysis of U-87MG cells using Anti-MIF Antibody (A309405) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-MIF Antibody (A309405)? Please let us know so that we can list the citation on this page.