Mouse monoclonal [NYRmI-A] antibody to MHC Class II I-A (FITC).
Specificity
This antibody recognises most MHC class II haplotypes (b,f,p,q,r,s,u,v), but weak against I-Ak.
Applications
Cytotoxic Assay, Flow Cytometry
Dilutions
FC: 10ml/1,000,000 cells.
Reactivity
Mouse
Immunogen
Purified mouse LN B cells (C3H anti-C57Bl6).
Host
Mouse
Clonality
Monoclonal
Clone ID
NYRmI-A
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Ion exchange chromatography.
Concentration
1mg/ml
Product Form
Lyophilized
Reconstitution
Reconstitute with sterile water. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Formulation
Lyophilized from Phosphate Buffered Saline.
Storage
Shipped at ambient temperature. Lyophilized: Store at 2-8°C. Reconstituted: Aliquot and store at -20°C long term or use within a month at 4°C. Avoid freeze/thaw cycles.
