Anti-mH2A1 Antibody (A15507)

Name: Anti-mH2A1 Antibody
Brand: Antibodies.com
SKU: A15507
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to mH2A1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Knockout Validated Antibody

Specifications

Name

Anti-mH2A1 Antibody

Description

Rabbit polyclonal antibody to mH2A1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human macroH2A.1 (NP_613258.2).

Sequence

KLEAIITPPPAKKAKSPSQKKPVSKKAGGKKGARKSKKKQGEVSKAASADSTTEGTPADGFTVLSTKSLFLGQKLNLIHSEISNLAGFEVEAIINPTNADIDLKDDLGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAVSAGHGLPAKFVIHCNSPVWGADKCEELLEKTVKNCLALADDKKLKSIAFPSIGSGRNGFPKQTAAQLILKAISSYFVSTMSSSIKTVYFVLFDSESIGIYVQEMAKLDAN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Core histone macro h2a.1, Core histone macro-H2A.1, H2A histone family member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2afy, H2AY_HUMAN, Histone H2A.Y, Histone macroH2A1, Histone macroH2A1.1, Histone macroH2A1.2, macroH2A.1, Macroh2a1, MACROH2A1.1, MacroH2A1.2, Medulloblastoma antigen MU MB 50.205, Medulloblastoma antigen MU-MB-50.205, mH2a

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-mH2A1 Antibody (A15507)

Western blot analysis of extracts from normal (control) and macroH2A. 1 Rabbit polyclonal antibody knockout (KO) 293T cells, using Anti-mH2A1 Antibody (A15507) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-mH2A1 Antibody (A15507)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-mH2A1 Antibody (A15507) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-mH2A1 Antibody (A15507)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using Anti-mH2A1 Antibody (A15507) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-mH2A1 Antibody (A15507)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-mH2A1 Antibody (A15507) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.