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Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340)

Western Blot - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) - Antibodies.com Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) - Antibodies.com Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Metabotropic Glutamate Receptor 5 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Metabotropic Glutamate Receptor 5 Antibody
Description
Rabbit polyclonal antibody to Metabotropic Glutamate Receptor 5.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 430-510 of human GRM5 (NP_001137303.1).
Sequence
KPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKSNIIRSV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
150 kDa / 300 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Glutamate receptor metabotropic 5, GPRC1E, Grm5, GRM5_HUMAN, Metabotropic glutamate receptor 5 variant F, Metabotropic glutamate receptor 5 variant G, Metabotropic glutamate receptor 5 variant H, mGlu5, mGluR5, PPP1R86, Protein phosphatase 1 regulatory subunit 86
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340)
Western blot analysis of extracts of various cell lines, using Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340)
Immunofluorescence analysis of mouse brain cells using Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340)
Immunofluorescence analysis of rat brain cells using Anti-Metabotropic Glutamate Receptor 5 Antibody (A16340) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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