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Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389)

Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389) - Antibodies.com Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC54652] antibody to Met (c-Met) (phospho Tyr1234 + Tyr1235) for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652]
Description
Rabbit monoclonal [ARC54652] antibody to Met (c-Met) (phospho Tyr1234 + Tyr1235).
Applications
WB
Dilutions
WB: 1:1,000-1:5,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around Y1234 & Y1235 of human Phospho-Met-Y1234/Y1235 (NP_000236.2).
Sequence
KEYYSV
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC54652
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
145 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AUTS9, c met, D249, Hepatocyte growth factor receptor, HGF, HGF receptor, HGF/SF receptor, HGFR, MET, Met proto oncogene tyrosine kinase, MET proto oncogene, receptor tyrosine kinase, Met proto-oncogene, Met proto-oncogene (hepatocyte growth factor receptor), Met protooncogene, MET_HUMAN, Oncogene MET, Par4, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389)
Western blot analysis of various lysates, using Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389) at 1:2,000 dilution. HeLa cells were treated by HGF (40 ng/ml) at 37°C for 10 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389)
Western blot analysis of various lysates, using Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody [ARC54652] (A306389) at 1:2,000 dilution. C2C12 and C6 cells were treated by HGF (40 ng/ml) at 37°C for 10 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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