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Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody (A11011)

Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody (A11011) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to Met (c-Met) (phospho Tyr1234 + Tyr1235) for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody
Description
Rabbit polyclonal antibody to Met (c-Met) (phospho Tyr1234 + Tyr1235).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
A synthetic phosphorylated peptide around Y1234 & Y1235 of human MET (NP_000236.2).
Sequence
KEYYSV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
145 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AUTS9, c met, D249, Hepatocyte growth factor receptor, HGF, HGF receptor, HGF/SF receptor, HGFR, MET, Met proto oncogene tyrosine kinase, MET proto oncogene, receptor tyrosine kinase, Met proto-oncogene, Met proto-oncogene (hepatocyte growth factor receptor), Met protooncogene, MET_HUMAN, Oncogene MET, Par4, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody (A11011) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody (A11011)
Western blot analysis of extracts of HeLa cells, using Anti-Met (c-Met) (phospho Tyr1234 + Tyr1235) Antibody (A11011) at 1:1,000 dilution. HeLa cells were treated by HGF (40 ng/ml) at 37°C for 10 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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