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Anti-Mesothelin Antibody (A92393)

Western Blot - Anti-Mesothelin Antibody (A92393) - Antibodies.com Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.com Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.com Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.com Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Mesothelin for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-Mesothelin Antibody
Description
Rabbit polyclonal antibody to Mesothelin.
Applications
WB, IHC
Dilutions
WB: 1:1,000-1:5,000, IHC: 1:50-1:200
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2).
Sequence
EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
46 - 48 kDa / 70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CAK 1, CAK1, CAK1 antigen, cleaved form, Megakaryocyte potentiating factor, Mesothelin isoform 1 precursor, MPF, Msln, MSLN_HUMAN, Pre pro megakaryocyte potentiating factor, Pre-pro-megakaryocyte-potentiating factor, SMR, SMRP, Soluble MPF mesothelin related protein
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Mesothelin Antibody (A92393) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Mesothelin Antibody (A92393)
Western blot analysis of extracts of OVCAR3 cells, using Anti-Mesothelin Antibody (A92393) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Mesothelin Antibody (A92393)
Immunohistochemistry analysis of paraffin-embedded human lung cancer (200X) using Anti-Mesothelin Antibody (A92393) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Mesothelin Antibody (A92393)
Immunohistochemistry analysis of paraffin-embedded human lung cancer (400X) using Anti-Mesothelin Antibody (A92393) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Mesothelin Antibody (A92393)
Immunohistochemistry analysis of paraffin-embedded human tonsil (200X) using Anti-Mesothelin Antibody (A92393) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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