Anti-Melanoma gp100 Antibody (A249977)

Anti-Melanoma gp100 Antibody

Rabbit polyclonal antibody to Melanoma gp100.

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments. Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml

Human

Recombinant full-length human SILV protein.

Rabbit

Polyclonal

IgG

Unconjugated

200 µg/ml

26-100 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Melanoma gp100 Antibody - BSA and Azide free (A253157).

95 kDa melanocyte specific secreted glycoprotein, 95 kDa melanocyte-specific secreted glycoprotein, D12S53E, gp100, M-beta, ME20, ME20 M/ME20 S, ME20-M, ME20-S, ME20M, ME20M/ME20S, ME20S, Melanocyte lineage specific antigen GP100, Melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocyte protein Pmel 17 precursor, Melanocytes lineage-specific antigen GP100, Melanoma associated ME20 antigen, Melanoma-associated ME20 antigen, Melanosomal matrix protein 17, Melanosomal matrix protein17, P1, p100, p26, PMEL, PMEL 17, PMEL17, PMEL_HUMAN, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SI, SIL, SILV, Silver (mouse homolog) like, Silver homolog, Silver locus protein homolog, Silver, mouse, homolog of