Anti-MEK1 (phospho Ser221) Antibody (A16426)

Name: Anti-MEK1 (phospho Ser221) Antibody
Brand: Antibodies.com
SKU: A16426
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to MEK1 (phospho Ser221) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-MEK1 (phospho Ser221) Antibody

Description

Rabbit polyclonal antibody to MEK1 (phospho Ser221).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S221 of human MEK1 (NP_002746.1).

Sequence

ANSFV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1_HUMAN, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-MEK1 (phospho Ser221) Antibody (A16426)

Western blot analysis of extracts of C6 cells, using Anti-MEK1 (phospho Ser221) Antibody (A16426) at 1:1,000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-MEK1 (phospho Ser221) Antibody (A16426)

Western blot analysis of extracts of HeLa cells, using Anti-MEK1 (phospho Ser221) Antibody (A16426) at 1:1,000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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