Anti-MEK1 Antibody (A92940)

Name: Anti-MEK1 Antibody
Brand: Antibodies.com
SKU: A92940
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to MEK1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-MEK1 Antibody

Description

Rabbit polyclonal antibody to MEK1.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:3,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK1 (NP_002746.1).

Sequence

MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1_HUMAN, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-MEK1 Antibody (A92940)

Western blot analysis of extracts of various cell lines, using Anti-MEK1 Antibody (A92940) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Western Blot - Anti-MEK1 Antibody (A92940)

Western blot analysis of extracts from normal (control) and MEK1 knockout (KO) HeLa cells, using Anti-MEK1 Antibody (A92940) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Immunohistochemistry - Anti-MEK1 Antibody (A92940)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-MEK1 Antibody (A92940) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MEK1 Antibody (A92940)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-MEK1 Antibody (A92940) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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