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MEF2B Cell Based ELISA Kit (A103474)

Western Blot - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.com Protocol Diagram - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.com Protocol Summary - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.com Western Blot - Control Antibody - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.com
$550
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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Email
orders@antibodies.com

Name
MEF2B Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This MEF2B Cell Based ELISA Kit allows for the detection of MEF2B and the effects that certain stimulation conditions have on MEF2B expression in different cell lines. Qualitative determination of MEF2B concentration is achieved by an indirect ELISA format. In essence, the MEF2B is captured by Anti-MEF2B Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MEF2B Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
MADS box transcription enhancer factor 2, MDS1 EVI1, MEF2B_HUMAN, Myocyte enhancer factor 2B, Myocyte specific enhancer factor 2B, Myocyte-specific enhancer factor 2B, PRDM3, RSRFR2, Serum response factor like protein 2, Serum response factor-like protein 2, XMEF2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.comEnlarge Image
Figure 1: Western Blot - MEF2B Cell Based ELISA Kit (A103474)
Western blot analysis of extracts from HT-29 cells, using Anti-MEF2B Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.comEnlarge Image
Figure 2: MEF2B Cell Based ELISA Kit (A103474)
A diagram showing the protocol for MEF2B Cell Based ELISA Kit.
Protocol Summary - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.comEnlarge Image
Figure 3: MEF2B Cell Based ELISA Kit (A103474)
A summary of the protocol for MEF2B Cell Based ELISA Kit.
Western Blot - Control Antibody - MEF2B Cell Based ELISA Kit (CB5952) - Antibodies.comEnlarge Image
Figure 4: Western Blot - MEF2B Cell Based ELISA Kit (A103474)
The mouse monoclonal antibody to GAPDH is used as a positive control inMEF2B Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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