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Anti-MED25 Antibody (A91199)

Western Blot - Anti-MED25 Antibody (A91199) - Antibodies.com Immunofluorescence - Anti-MED25 Antibody (A91199) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to MED25 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-MED25 Antibody
Description
Rabbit polyclonal antibody to MED25.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2).
Sequence
PYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
78 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ACID1, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, Mediator of RNA polymerase II transcription subunit 25, p78, PTOV2
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-MED25 Antibody (A91199) - Antibodies.comEnlarge Image

Western Blot - Anti-MED25 Antibody (A91199)

Western blot analysis of extracts of various cell lines, using Anti-MED25 Antibody (A91199) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-MED25 Antibody (A91199) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-MED25 Antibody (A91199)

Immunofluorescence analysis of U-2 OS cells using Anti-MED25 Antibody (A91199) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-MED25 Antibody (A91199)? Please let us know so that we can list the citation on this page.

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