Anti-MED12 Antibody (A295806)

Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Anti-MED12 Antibody (A295806) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Samples: Whole cell lysate (10 µg) from HeLa, A-549, GaMG, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Anti-MED12 Antibody (A295806) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-MED12 Antibody (A295806) was used for IP at 6 µg per reaction. MED12 was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of MED12. For blotting immunoprecipitated MED12, Anti-MED12 Antibody (A295806) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Sample: FFPE section of mouse renal cell carcinoma. Antibody: Anti-MED12 Antibody (A295806) was used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.