Rabbit polyclonal antibody to MDM4 (Phospho-Ser367)
Specificity
The antibody detects endogenous levels of MDM4 only when phosphorylated at serine 367.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 367 (T-I-S(p)-A-P) derived from Human MDM4.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
Double minute 4 protein, Mdm2-like p53-binding protein, p53-binding protein Mdm4, Protein Mdm4, Protein Mdmx
Western blot analysis of extracts from HeLa cells, treated with calyculin A (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody #12141. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody #12141. The picture on the right is treated with the synthesized peptide.
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