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MDM4 Cell Based ELISA Kit (A103196)

Western Blot - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.com Protocol Diagram - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.com Protocol Summary - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.com Western Blot - Control Antibody - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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Name
MDM4 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This MDM4 Cell Based ELISA Kit allows for the detection of MDM4 and the effects that certain stimulation conditions have on MDM4 expression in different cell lines. Qualitative determination of MDM4 concentration is achieved by an indirect ELISA format. In essence, the MDM4 is captured by Anti-MDM4 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MDM4 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
DKFZp781B1423, Double minute 4, Double minute 4 human homolog of p53 binding protein, Double minute 4 protein, HDMX, MDM 4, Mdm2 like p53 binding protein, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog mouse, Mdm4 protein, MDM4 related protein 1, Mdm4 transformed 3T3 cell double minute 4, Mdm4 transformed 3T3 cell double minute 4 p53 binding protein, Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse, MDM4_HUMAN, MDMX, Mdmx protein, MGC132766, Mouse double minute 4 homolog, Mouse double minute 4 human homolog of p53 binding protein, MRP 1, MRP1, p53 binding protein, p53 BINDING PROTEIN MDM4, p53-binding protein Mdm4, Protein Mdm4, Protein Mdmx
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.comEnlarge Image
Figure 1: Western Blot - MDM4 Cell Based ELISA Kit (A103196)
Western blot analysis of extracts from rat muscle cells, using Anti-MDM4 (Ab-367) Antibody.
Protocol Diagram - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.comEnlarge Image
Figure 2: MDM4 Cell Based ELISA Kit (A103196)
A diagram showing the protocol for MDM4 Cell Based ELISA Kit.
Protocol Summary - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.comEnlarge Image
Figure 3: MDM4 Cell Based ELISA Kit (A103196)
A summary of the protocol for MDM4 Cell Based ELISA Kit.
Western Blot - Control Antibody - MDM4 Cell Based ELISA Kit (CB5437) - Antibodies.comEnlarge Image
Figure 4: Western Blot - MDM4 Cell Based ELISA Kit (A103196)
The mouse monoclonal antibody to GAPDH is used as a positive control inMDM4 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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