Rabbit polyclonal antibody to MDM2 (phospho-Ser166)
Specificity
The antibody detects endogenous level of MDM2 only when phosphorylated at serine 166.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from Human MDM2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166) Antibody #11550 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-MDM2 Antibody [SMP14] (A249312) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249312_1.jpg?profile=product_alternative)
![Western Blot - Anti-MDM2 Antibody [SMP14] - BSA and Azide free (A252492) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252492_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-MDM2 (phospho Ser166) Antibody [ARC2909] (A309403) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309403_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-MDM2 Antibody [MDM2/2414] (A249313) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249313_1.jpg?profile=product_alternative)