Figure 1: Western Blot - Anti-MDH2 Antibody (A85260)
MDH2 expression in HeLa (A), HepG2 (B), and K562 (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-MDH2 Antibody (A85260) at 0.03µg/ml and detected by chemiluminescence.
Figure 2: Western Blot - Anti-MDH2 Antibody (A85260)
MDH2 expression in Human Heart (A) and Liver (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-MDH2 Antibody (A85260) at 0.03µg/ml and detected by chemiluminescence.
MDH2 expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-MDH2 Antibody (A85260) at 5µg/ml and revealed with alkaline phosphatase (AP).
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