Anti-MDC1 Antibody [P2B11] (A304945)

Name: Anti-MDC1 Antibody [P2B11]
Brand: Antibodies.com
SKU: A304945
Price: 465.0 USD
Availability: InStock

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$465

Mouse monoclonal [P2B11] antibody to MDC1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MDC1 Antibody [P2B11]

Description

Mouse monoclonal [P2B11] antibody to MDC1.

Specificity

This antibody recognizes MDC1 at and around the N-terminus.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Bovine, Chimpanzee

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Host

Mouse

Clonality

Monoclonal

Clone ID

P2B11

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~184 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Homologue to Drosophila photoreceptor protein calphotin, MDC 1, MDC1_HUMAN, Mediation of DNA damage checkpoint 1, Mediator of DNA damage checkpoint 1, Mediator of DNA damage checkpoint protein 1, NFBD 1, NFBD1, Nuclear factor with BRCT domains 1, Nuclear Factor with BRCT Domains Protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP: IgG at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 2: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: mouse Cortex. Lane 3: mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 3: Immunofluorescence - Anti-MDC1 Antibody [P2B11] (A304945)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-MDC1 Antibody [P2B11] (A304945), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.

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