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Anti-MCM5 Antibody [ARC1239] (A305293)

Western Blot - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.com Immunohistochemistry - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.com Immunohistochemistry - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.com Western Blot - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC1239] antibody to MCM5 for WB, IHC and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-MCM5 Antibody [ARC1239]
Description
Rabbit monoclonal [ARC1239] antibody to MCM5.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 635-734 of human MCM5 (P33992).
Sequence
ADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1239
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
82 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CDC 46, CDC46, CDC46 homolog, Cell division cycle 46, DNA replication licensing factor, DNA replication licensing factor MCM 5, DNA replication licensing factor MCM5, MCM 5, MCM5_HUMAN, MGC5315, Minichromosome maintenance complex component 5, Minichromosome maintenance deficient (S. cerevisiae) 5, Minichromosome maintenance deficient 5, Minichromosome maintenance deficient protein 5, P1 CDC46, P1-CDC46
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-MCM5 Antibody [ARC1239] (A305293)
Western blot analysis of extracts of various cell lines, using Anti-MCM5 Antibody [ARC1239] (A305293) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-MCM5 Antibody [ARC1239] (A305293)
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-MCM5 Antibody [ARC1239] (A305293) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-MCM5 Antibody [ARC1239] (A305293)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-MCM5 Antibody [ARC1239] (A305293) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot - Anti-MCM5 Antibody [ARC1239] (A305293) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-MCM5 Antibody [ARC1239] (A305293)
Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-MCM5 Antibody [ARC1239] (A305293). This Western blot was performed on the immunoprecipitate using Anti-MCM5 Antibody [ARC1239] (A305293) at a dilution of 1:1000.
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