Synthetic peptide within amino acids 1-50 of human MCM5 (NP_006730. 2).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
CDC46, CDC46 homolog, DNA replication licensing factor MCM5, P1-CDC46
Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Anti-MCM5 Antibody (A295329) was used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.
Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Anti-MCM5 Antibody (A295329) was used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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