Synthetic peptide corresponding to the internal region of human MBD2 (Isoform 1).
Sequence
C-RNDPLNQNKGKPDLN
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
48-50 kDa
Predicted MW
43.3kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Demethylase, DMTase, MBD 2, MBD2a, MBD2_HUMAN, Methyl CpG binding domain protein 2, Methyl CpG binding protein MBD2, Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, NY CO 41
Figure 1: Western Blot - Anti-MBD2 Antibody (A82510)
MBD2 expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-MBD2 Antibody (A82510) at 0.3µg/ml and detected by chemiluminescence.
MBD2 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-MBD2 Antibody (A82510) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
MBD2 expression in Human Adrenal Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-MBD2 Antibody (A82510) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
MBD2 expression in Human Skin analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-MBD2 Antibody (A82510) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
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