Anti-MARK4 Antibody (A82518)

Name: Anti-MARK4 Antibody
Brand: Antibodies.com
SKU: A82518
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to MARK4 for ELISA, WB and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MARK4 Antibody

Description

Goat polyclonal antibody to MARK4.

Applications

ELISA, WB, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.3-1µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the C terminal region of human MARK4.

Sequence

IKRKPPPGCSDSPGV

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

70 kDa

Predicted MW

75.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

FLJ90097, KIAA1860, MAP / microtubule affinity regulating kinase 4, MAP / microtubule affinity regulating kinase 4L, MAP/microtubule affinity regulating kinase 4, MAP/microtubule affinity regulating kinase like 1, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1, MARK 4, MARK 4L, MARK L1, MARK4 serine / threonine protein kinase, MARK4 serine/threonine protein kinase, MARK4S, MARK4_HUMAN, MARKL 1, MARKL1, Nbla00650, Putative protein product of Nbla00650

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MARK4 Antibody (A82518)

MARK4 expression in Human Cerebellum lysate (A) + peptide (B), Mouse Brain lysate (C) + peptide (D) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-MARK4 Antibody (A82518) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-MARK4 Antibody (A82518)

MARK4 expression in U251 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-MARK4 Antibody (A82518) at 1µg/ml and detected by chemiluminescence.

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Figure 3: Flow Cytometry - Anti-MARK4 Antibody (A82518)

MARK4 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-MARK4 Antibody (A82518) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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