Anti-MAP2 Antibody (A85363)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-MAP2 Antibody, at a dilution of 1:10,000, in red, and Anti-Tau, MAPT Antibody (A85416 | 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Anti-MAP2 Antibody stains dendrites and perikarya of neurons, while the Anti-Tau, MAPT Antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.

View of mixed neuron/glial cultures stained with Anti-MAP2 Antibody (red). The perikarya and dendrites of neurons are strongly and specifically stained with the Anti-MAP2 Antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.

Western blot analysis of whole brain tissue lysates using Anti-MAP2 Antibody, at a dilution of 1:50,000, in green,: [Lane 1] protein standard (red), [Lane 2] adult rat brain, [Lane 3] embryonic E20 rat brain, [Lane 4] adult mouse brain. Strong band at about 280 kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations. This antibody was raised against the “projection domain” sequences which are not found in MAP2C and MAP2D, and so this antibody does not recognize these isotypes.

High magnification stacked confocal image of rat cerebellar molecular layer at top and granular layer below, stained with Anti-Iba1 Antibody (A104332), at a dilution of 1:1,000, in green. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. The processes of Purkinje cells and the perikarya of granule cells are revealed with Anti-MAP2 Antibody (A85363), at a dilution of 1:5,000, in red. Nuclear DNA is shown with DAPI stain in blue.