Anti-Major Vault Protein Antibody [SPM280] (A250816)

Anti-Major Vault Protein Antibody [SPM280]

Mouse monoclonal [SPM280] antibody to Major Vault Protein.

This antibody recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Proteins precipitated from human breast cancer MCF-7 cells.

Mouse

Monoclonal

SPM280

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

104-110 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Major Vault Protein Antibody [SPM280] - BSA and Azide free (A253996).

LRP, Lung resistance related protein, Lung resistance-related protein, Major vault protein, rat, homolog of, MVP, MVP_HUMAN, testicular secretory protein Li 30, VAULT 1, VAULT1