Anti-Macrophages/Monocytes/Granulocytes Antibody [MAC387] (A281028)

Anti-Macrophages/Monocytes/Granulocytes Antibody [MAC387]

Mouse monoclonal [MAC387] antibody to Macrophages/Monocytes/Granulocytes.

This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.

IHC-P, Flow Cytometry, IHC-Fr

FC 1: 1:50 - 1:100, IHC-Fr: 1:100 - 1:200, IHC-P 2: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human, Horse, Pig, Dog, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Cat, Cynomolgus monkey, Rhesus Monkey, Goat, Fallow deer, Pygmy hippopotamus, Mink, Marmoset

Human monocytes.

Mouse

Monoclonal

MAC387

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.