Anti-Macrophage L1 Protein Antibody [SPM281] (A249926)

Anti-Macrophage L1 Protein Antibody [SPM281]

Mouse monoclonal [SPM281] antibody to Macrophage L1 Protein.

This antibody recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffers cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Baboon, Monkey, Bovine, Porcine, Goat, Horse, Feline, Canine, Rabbit, Guinea Pig, Rat, Mouse

Affinity purified monocyte membrane preparation.

Mouse

Monoclonal

SPM281

IgG1

Unconjugated

Protein A/G chromatography.

200 µg/ml

12-14 kDa (doublet)

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Macrophage L1 Protein Antibody [SPM281] - BSA and Azide free (A253106).