Anti-LRRK2 Antibody [S231B-34] (A305152)

Name: Anti-LRRK2 Antibody [S231B-34]
Brand: Antibodies.com
SKU: A305152
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S231B-34] antibody to LRRK2 for WB, ICC/IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-LRRK2 Antibody [S231B-34]

Description

Mouse monoclonal [S231B-34] antibody to LRRK2.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:100

Reactivity

Human, Rat

Immunogen

Fusion protein amino acids 841-960 of human LRRK2. 81% identical in mouse, 80% identical in rat. <30% identity with LRRK1..

Host

Mouse

Clonality

Monoclonal

Clone ID

S231B-34

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

>200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

augmented in rheumatoid arthritis 17, AURA17, Dardarin, Leucine rich repeat kinase 2, leucine rich repeat serine threonine protein kinase 2, Leucine-rich repeat serine/threonine-protein kinase 2, LRRK 2, LRRK2_HUMAN, PARK 8, PARK8, RIPK7, ROCO 2, ROCO2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-LRRK2 Antibody [S231B-34] (A305152)

Western blot analysis of rat brain membrane showing detection of >200 kDa LRRK2/Dardarin protein using Anti-LRRK2 Antibody [S231B-34] (A305152) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: >200 kDa.

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Figure 2: Immunofluorescence - Anti-LRRK2 Antibody [S231B-34] (A305152)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-LRRK2 Antibody [S231B-34] (A305152), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRRK2/Dardarin Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-LRRK2 Antibody [S231B-34] (A305152)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-LRRK2 Antibody [S231B-34] (A305152), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin Antibody (D) Composite.

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