Anti-LLGL1 Antibody (A307538)

Name: Anti-LLGL1 Antibody
Brand: Antibodies.com
SKU: A307538
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to LLGL1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-LLGL1 Antibody

Description

Rabbit polyclonal antibody to LLGL1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human LLGL1 (NP_004131.3).

Sequence

MMKFRFRRQGADPQREKLKQELFAFNKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTGLHRDAATVTQMHFLTGQGRLLSLLDDSSLHLWEIVHHNGCAHLEEALSFQLPSRPGFDGASAPLSLTRVTVVLLVAAGDIAALGTEGSSVFFLDVTTLTLLEGQTLAPGEVLRSVPDDYR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

115 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DLG4, DLG4, formerly, HUGL, Hugl 1, Hugl-1, HUGL1, Human homolog to the D-lgl gene protein, L2GL1_HUMAN, Lethal giant larvae homolog 1, Lethal giant larvae homolog 1 (Drosophila), Lethal(2) giant larvae protein homolog 1, LLGL, Ly 49G2, Ly49G2, Mgl1, Mlgl

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-LLGL1 Antibody (A307538)

Western blot analysis of extracts of various cell lines, using Anti-LLGL1 Antibody (A307538) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-LLGL1 Antibody (A307538)

Western blot analysis of extracts of Rat testis, using Anti-LLGL1 Antibody (A307538) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-LLGL1 Antibody (A307538)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-LLGL1 Antibody (A307538) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-LLGL1 Antibody (A307538)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-LLGL1 Antibody (A307538) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.