Principle of Assay
Distinguishing between live and dead cells is very important for investigation of growth control and cell death. This kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells.