Anti-Lipoamide Dehydrogenase Antibody (A14737)

Name: Anti-Lipoamide Dehydrogenase Antibody
Brand: Antibodies.com
SKU: A14737
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Lipoamide Dehydrogenase for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Lipoamide Dehydrogenase Antibody

Description

Rabbit polyclonal antibody to Lipoamide Dehydrogenase.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLDH/DLDH/DLDH/DLD (NP_000099.2).

Sequence

ADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dehydrogenase complex, E3 component, Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, dld, DLDD, DLDH, DLDH_HUMAN, E3, E3 branched chain aplha-keto acid, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, GCSL, Glycine cleavage system L protein, Glycine cleavage system protein L, LAD, Lipoamide reductase, Lipoyl dehydrogenase, mitochondrial, OTTHUMP00000206744, OTTHUMP00000206746, OTTHUMP00000206748, OTTHUMP00000206749, PHE 3, PHE3, Pyruvate dehydrogenase component E3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Lipoamide Dehydrogenase Antibody (A14737)

Western blot analysis of extracts of various cell lines, using Anti-Lipoamide Dehydrogenase Antibody (A14737) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody (A14737)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Lipoamide Dehydrogenase Antibody (A14737) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody (A14737)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Lipoamide Dehydrogenase Antibody (A14737) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody (A14737)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Lipoamide Dehydrogenase Antibody (A14737) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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