Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CSDD1, FLJ12457, LIN 28, Lin 28 homolog A (C. elegans), Lin-28A, LIN28, LN28A_HUMAN, Protein lin-28 homolog A, ZCCHC1, zinc finger CCHC domain containing 1, Zinc finger CCHC domain-containing protein 1
Figure 1: Western Blot - Anti-Lin28A Antibody (A11914)
Western blot analysis of extracts of T47D cells, using Anti-Lin28A Antibody (A11914) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human testicular seminoma using Anti-Lin28A Antibody (A11914) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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