This antibody recognises CD85j, a member of the leukocyte immunoglobulin-like receptor (LIR) family.CD85j is a receptor for MHC Class I molecules and ligand binding results in inhibitory signals and down-regulation of the immune response.CD85j is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells, T-cells and natural killer (NK) cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Immunogen
Monocyte derived dendritic cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
4F9
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD85j antibody, clone 4F9 recognizes CD85j, a member of the leukocyte immunoglobulin-like receptor (LIR) family.CD85j is a receptor for MHC Class I molecules and ligand binding results in inhibitory signals and down-regulation of the immune response.CD85j is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells, T-cells and natural killer (NK) cells.
Synonyms
CD85 antigen-like family member J, CD85j, ILT-2, ILT2, Immunoglobulin-like transcript 2, Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, LIR1, MIR-7, MIR7, Monocyte/macrophage immunoglobulin-like receptor 7