Anti-LIF Antibody (A121175)

Name: Anti-LIF Antibody
Brand: Antibodies.com
SKU: A121175
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to LIF for ELISA, Flow Cytometry and IHC.

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Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Specifications

Name

Anti-LIF Antibody

Description

Goat polyclonal antibody to LIF.

Applications

ELISA, Flow Cytometry, IHC

Dilutions

ELISA: 1:128,000, IHC: 6µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human LIF.

Sequence

RLCSKYHVGHVDVT

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CDF, Cholinergic Differentiation Factor, D factor, DIA, Differentiation inducing factor, differentiation inhibitory activity, Differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, Hepatocyte stimulating factor III, HILDA, Human interleukin in DA cells, Leukemia inhibitory factor, LIF_HUMAN, Melanoma derived LPL inhibitor, Melanoma-derived LPL inhibitor, MLPLI

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Flow Cytometry - Anti-LIF Antibody (A121175)

LIF expression in HepG2 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-LIF Antibody (A121175) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 2: Immunohistochemistry - Anti-LIF Antibody (A121175)

LIF expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-LIF Antibody (A121175) at 6µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-LIF Antibody (A121175)

Negative control for LIF expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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A283775

Anti-LIF Antibody (A121175)

Goat polyclonal antibody to LIF for ELISA, Flow Cytometry and IHC.

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