Synthetic peptide corresponding to the internal region of human LGN.
Sequence
C-NRLKGKKYKTNSSTK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DFNB82, G protein signalling modulator 2, G protein signalling modulator 2 (AGS3 like C. elegans), G-protein-signaling modulator 2, GPSM2, GPSM2_HUMAN, HGNC:29501, LGN protein, Mosaic protein LGN, Pins
LGN expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-LGN Antibody (A84294) at 5µg/ml and revealed with alkaline phosphatase (AP).
LGN expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-LGN Antibody (A84294) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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