Anti-LGI1 Antibody [S283-7] (A305011)

Name: Anti-LGI1 Antibody [S283-7]
Brand: Antibodies.com
SKU: A305011
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S283-7] antibody to LGI1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-LGI1 Antibody [S283-7]

Description

Mouse monoclonal [S283-7] antibody to LGI1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4

Host

Mouse

Clonality

Monoclonal

Clone ID

S283-7

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADLTE, ADPAEF, ADPEAF, EPITEMPIN, Epitempin 1, Epitempin-1, EPT, ETL1, IB1099, leucine rich glioma inactivated 1, Leucine rich glioma-inactivated protein 1, Leucine-rich glioma-inactivated protein 1, LGI1_HUMAN, OTTHUMP00000020121, OTTHUMP00000020122

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-LGI1 Antibody [S283-7] (A305011)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-LGI1 Antibody [S283-7] (A305011), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LGI1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-LGI1 Antibody [S283-7] (A305011)

Western blot analysis of rat brain membrane showing detection of ~60 kDa LGI1 protein using Anti-LGI1 Antibody [S283-7] (A305011) at 1:1,000 for 1 hour at room temperature. Load: 10 µg. The secondary antibody used was Goat Anti-Mouse HRP at 1:200 for 1 hour at room temperature. Predicted/Observed Size: ~60 kDa.

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Figure 3: Immunofluorescence - Anti-LGI1 Antibody [S283-7] (A305011)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-LGI1 Antibody [S283-7] (A305011), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LGI1 Antibody. (D) Composite.

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