Anti-Leptin Receptor Antibody (A83422)

Name: Anti-Leptin Receptor Antibody
Brand: Antibodies.com
SKU: A83422
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Leptin Receptor for ELISA, WB, IHC and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Specifications

Name

Anti-Leptin Receptor Antibody

Description

Goat polyclonal antibody to Leptin Receptor.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 1-2µg/ml, IHC: 4-6µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region of human Leptin Receptor.

Sequence

C-TQDDIEKHQSDAG

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

140-150 kDa

Predicted MW

132kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CD 295, CD295, CD295 antigen, Db, Fa, HuB219, LEP R, LEP-R, LEPR, LEPRD, LEPR_HUMAN, Leptin receptor fatty, Leptin receptor gene related protein, Leptin receptor precursor, OB R gene related protein, OB receptor, OB-R, OB-RGRP, obl, Obr

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in Human Cerebellum (A), Mouse Foetal Brain (B), and Rat Brain (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Leptin Receptor Antibody (A83422) at 1.75µg/ml (A) or 1.5µg/ml (B-C) and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Leptin Receptor Antibody (A83422) at 6µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-Leptin Receptor Antibody (A83422)

Negative control for Leptin Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 4: Flow Cytometry - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Leptin Receptor Antibody (A83422) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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