Anti-Leptin Antibody (A11435)

Name: Anti-Leptin Antibody
Brand: Antibodies.com
SKU: A11435
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Leptin for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Leptin Antibody

Description

Rabbit polyclonal antibody to Leptin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Leptin (NP_000221.1).

Sequence

MHWGTLCGFLWLWPYLFYVQAVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISND

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLJ94114, LEP, LEPD, Leptin (murine obesity homolog), Leptin (obesity homolog, mouse), Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, LEP_HUMAN, OB, Obese protein, Obese, mouse, homolog of, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS, OTTHUMP00000212285

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Leptin Antibody (A11435)

Western blot analysis of extracts of various cell lines, using Anti-Leptin Antibody (A11435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-Leptin Antibody (A11435)

Immunofluorescence analysis of HepG2 cells using Anti-Leptin Antibody (A11435) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Leptin Antibody (A11435)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Leptin Antibody (A11435) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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