Anti-Legionella pneumophila Aptamer [R10C5] (Biotin) (A321212)

Freight Charges
Lead Time
30-45 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Legionella pneumophila Aptamer [R10C5] (Biotin)
Description
Synthetic DNA aptamer to Legionella pneumophila [R10C5] (Biotin).
Aptamer ID
R10C5
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Phosphate Buffered Saline, 1% Bovine Serum Albumin, 0.1mg/ml Salmon Sperm, and 0.05% Tween 20. Binding Temperature: 25°C.
Sequence
5'-dGpdCpdApdApdTpdGpdGpdTpdApdCpdGpdGpdTpdApdCpdTpdTpdCpdCpdGpdGpdApdCpdApdGpdTpdGpdCpdTpdGpdApdApdApdApdCpdTpdGpdTpdGpdApdCpdCpdCpdCpdCpdCpdApdApdApdApdGpdTpdGpdCpdApdCpdGpdCpdTpdApdCpdTpdTpdTpdGpdCpdTpdApdAp-3'
Aptamer Length
69 nt
Aptamer Molecular Weight (Da)
21,213.8 Da
Aptamer Affinity
Affinity (Kd) = 116 nM
Extinction Coefficient
658800 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Legionella pneumophila Aptamer [R10C5] (Biotin) (A321212) is a replica of the aptamer described in PMID: 32499557.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-Legionella pneumophila Aptamer [R10C5] (Biotin) (A321212)? Please let us know so that we can list the citation on this page.
Top