Anti-Lck (phospho Tyr394) Antibody (A12463)

Name: Anti-Lck (phospho Tyr394) Antibody
Brand: Antibodies.com
SKU: A12463
Price: 450.0 USD
Availability: InStock

Enlarge Image

$450

Rabbit polyclonal antibody to Lck (phospho Tyr394) for WB.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Plasma Membrane Marker

Specifications

Name

Anti-Lck (phospho Tyr394) Antibody

Description

Rabbit polyclonal antibody to Lck (phospho Tyr394).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around Y394 of human LCK (NP_005347.3).

Sequence

NEYTA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Enlarge Image

Western Blot - Anti-Lck (phospho Tyr394) Antibody (A12463)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Lck (phospho Tyr394) Antibody (A12463) at 1:1,000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Western Blot - Anti-Lck (phospho Tyr394) Antibody (A12463)

Western blot analysis of extracts of Jurkat cells, using Anti-Lck (phospho Tyr394) Antibody (A12463) at 1:1,000 dilution. Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Publishing research using Anti-Lck (phospho Tyr394) Antibody (A12463)? Please let us know so that we can list the citation on this page.