Anti-LC3A Antibody (A304958)

Name: Anti-LC3A Antibody
Brand: Antibodies.com
SKU: A304958
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to LC3A for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Autophagosome Marker

Specifications

Name

Anti-LC3A Antibody

Description

Rabbit polyclonal antibody to LC3A.

Specificity

Band at 50 kDa is LC3A complex.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, MAP1 light chain 3 like protein 1, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, Microtubule associated proteins 1A/1B light chain 3, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A and 1B, light chain 3, Microtubule-associated proteins 1A/1B light chain 3A, MLP3A_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-LC3A Antibody (A304958)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-LC3A Antibody (A304958), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Autophagosome, Cytoplasmic Vesicle. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LC3A Antibody (D) Composite.

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Figure 2: Western Blot - Anti-LC3A Antibody (A304958)

Western blot analysis of rat liver cell lysates showing detection of 14 kDa LC3A protein using Anti-LC3A Antibody (A304958) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 14 kDa.

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