Anti-Laminin beta 1 Antibody [ARC0985] (A306424)

Name: Anti-Laminin beta 1 Antibody [ARC0985]
Brand: Antibodies.com
SKU: A306424
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0985] antibody to Laminin beta 1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Laminin beta 1 Antibody [ARC0985]

Description

Rabbit monoclonal [ARC0985] antibody to Laminin beta 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1687-1786 of human Laminin beta 1 (P07942).

Sequence

KKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLLKDISQKVAVYSTCL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0985

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

LAMB1, Laminin B1 chain, Laminin subunit beta-1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Laminin beta 1 Antibody [ARC0985] (A306424)

Western blot analysis of extracts of Mouse heart, using Anti-Laminin beta 1 Antibody [ARC0985] (A306424) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-Laminin beta 1 Antibody [ARC0985] (A306424)

Western blot analysis of extracts of Rat lung, using Anti-Laminin beta 1 Antibody [ARC0985] (A306424) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Western Blot - Anti-Laminin beta 1 Antibody [ARC0985] (A306424)

Western blot analysis of extracts of HepG2 cells, using Anti-Laminin beta 1 Antibody [ARC0985] (A306424) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunofluorescence - Anti-Laminin beta 1 Antibody [ARC0985] (A306424)

Immunofluorescence analysis of C6 cells using Anti-Laminin beta 1 Antibody [ARC0985] (A306424) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).